Instructor Notes

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Introduction: Single-Cell Transcriptomics in Weakly Electric Fish

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Terminology note

Older literature (and Losilla & Gallant 2025) uses “pacemaker nucleus” for the small cells ventral to the relay nucleus that initiate each EOD command. Modern Carlson-lab papers (Fukutomi & Carlson 2023, Jarzyna & Carlson 2026) use “command nucleus (CN)” for the same population. They refer to the same structure — flag this for students if they see both terms across the reading list.

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11-Ketotestosterone Implantation in Mormyrid Electric Fish

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Examining EOD Duration from Raw Recordings

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Instructor Note

Ask learners what they notice about the waveform shape: there should be a positive peak (P1) followed by a negative trough (P2). This biphasic structure is the signature of mormyrid EODs. Testosterone extends the duration of P2, making the whole EOD longer.

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